Biochemistry MCQs 50 Score: 0 Attempted: 0/50 Subscribe 1. Which is the building block of proteins? (A) Fatty acids (B) Amino acids (C) Nucleotides (D) Monosaccharides 2. Which bond joins amino acids in a protein? (A) Hydrogen bond (B) Disulfide bond (C) Peptide bond (D) Ionic bond 3. The primary storage form of glucose in animals is: (A) Starch (B) Glycogen (C) Cellulose (D) Maltose 4. Which enzyme breaks down starch into maltose? (A) Amylase (B) Lipase (C) Pepsin (D) Trypsin 5. Which vitamin acts as a coenzyme in carboxylation reactions? (A) Vitamin A (B) Vitamin D (C) Vitamin K (D) Vitamin B12 6. The main nitrogenous waste product in birds is: (A) Urea (B) Uric acid (C) Ammonia (D) Creatinine 7. Which molecule is the universal energy currency of the cell? (A) GTP (B) ATP (C) NADH (D) FAD 8. The process of glucose breakdown to pyruvate is called: (A) Gluconeogenesis (B) Glycogenesis (C) Glycolysis (D) Glycogenolysis 9. Which element is a component of hemoglobin? (A) Copper (B) Iron (C) Zinc (D) Magnesium 10. The enzyme that converts fibrinogen to fibrin is: (A) Trypsin (B) Pepsin (C) Thrombin (D) Plasmin 11. DNA replication occurs during which phase of the cell cycle? (A) G1 phase (B) S phase (C) G2 phase (D) M phase 12. Which vitamin deficiency causes rickets? (A) Vitamin A (B) Vitamin B12 (C) Vitamin C (D) Vitamin D 13. The process of synthesis of glucose from non-carbohydrate sources is: (A) Glycolysis (B) Gluconeogenesis (C) Glycogenolysis (D) Lipolysis 14. The main function of lipase is: (A) Break down starch (B) Break down proteins (C) Break down fats (D) Break down nucleic acids 15. Which amino acid is essential for cats? (A) Glycine (B) Taurine (C) Alanine (D) Serine 16. The major plasma protein responsible for maintaining osmotic pressure is: (A) Globulin (B) Albumin (C) Fibrinogen (D) Hemoglobin 17. Which enzyme catalyzes the conversion of hydrogen peroxide to water and oxygen? (A) Peroxidase (B) Catalase (C) Oxidase (D) Reductase 18. The basic unit of nucleic acids is: (A) Amino acid (B) Nucleotide (C) Fatty acid (D) Monosaccharide 19. Which lipid is a major component of cell membranes? (A) Cholesterol (B) Phospholipid (C) Triglyceride (D) Steroid 20. The enzyme responsible for DNA unwinding during replication is: (A) Ligase (B) Helicase (C) Polymerase (D) Primase 21. Which coenzyme is derived from niacin? (A) FAD (B) NAD+ (C) CoA (D) TPP 22. The first step of the Krebs cycle involves: (A) Oxaloacetate + Acetyl-CoA β Citrate (B) Pyruvate β Lactate (C) Malate β Oxaloacetate (D) Citrate β Isocitrate 23. Which hormone promotes glycogen breakdown? (A) Insulin (B) Glucagon (C) Calcitonin (D) Somatostatin 24. The end product of anaerobic glycolysis in muscles is: (A) Pyruvate (B) Lactate (C) Acetyl-CoA (D) Ethanol 25. The enzyme that synthesizes RNA from DNA template is: (A) DNA polymerase (B) RNA polymerase (C) Ligase (D) Helicase 26. Which mineral is a cofactor for many enzymes and essential for insulin action? (A) Calcium (B) Magnesium (C) Zinc (D) Iron 27. The major site of protein digestion is: (A) Mouth (B) Stomach (C) Small intestine (D) Large intestine 28. The enzyme that converts angiotensin I to angiotensin II is: (A) Renin (B) ACE (Angiotensin-Converting Enzyme) (C) Aldosterone (D) Kallikrein 29. The genetic material in retroviruses is: (A) DNA (B) RNA (C) Protein (D) Peptide 30. Which vitamin functions as an antioxidant? (A) Vitamin D (B) Vitamin C (C) Vitamin K (D) Vitamin B6 31. The process of breaking down fatty acids for energy is: (A) Lipogenesis (B) Beta-oxidation (C) Ketogenesis (D) Gluconeogenesis 32. The enzyme that catalyzes the rate-limiting step in cholesterol synthesis is: (A) HMG-CoA synthase (B) HMG-CoA reductase (C) Acetyl-CoA carboxylase (D) Citrate lyase 33. Which molecule carries amino acids to the ribosome during protein synthesis? (A) mRNA (B) rRNA (C) tRNA (D) snRNA 34. The main function of chylomicrons is: (A) Carry cholesterol to tissues (B) Transport triglycerides from intestine to tissues (C) Transport glucose (D) Store fat in liver 35. The normal blood glucose level in most domestic animals is approximately: (A) 20β40 mg/dL (B) 50β70 mg/dL (C) 70β120 mg/dL (D) 150β200 mg/dL 36. The urea cycle occurs mainly in the: (A) Kidney (B) Liver (C) Pancreas (D) Small intestine 37. Which enzyme digests DNA and RNA? (A) Amylase (B) Nuclease (C) Protease (D) Lipase 38. The primary end product of purine metabolism in humans is: (A) Ammonia (B) Uric acid (C) Urea (D) Creatine 39. Which hormone promotes glucose uptake by cells? (A) Glucagon (B) Epinephrine (C) Insulin (D) Cortisol 40. The enzyme involved in lactose digestion is: (A) Lactase (B) Maltase (C) Sucrase (D) Amylase 41. The major component of bile salts is derived from: (A) Fatty acids (B) Cholesterol (C) Amino acids (D) Nucleic acids 42. The functional unit of a protein is determined by: (A) Primary structure (B) Secondary structure (C) Tertiary structure (D) Quaternary structure 43. Which vitamin is essential for collagen synthesis? (A) Vitamin A (B) Vitamin C (C) Vitamin D (D) Vitamin E 44. The enzyme that adds a new DNA strand during replication is: (A) DNA ligase (B) DNA polymerase (C) RNA polymerase (D) Helicase 45. The hormone that regulates basal metabolic rate is: (A) Insulin (B) Cortisol (C) Thyroxine (D) Epinephrine 46. The main storage form of fat in animals is: (A) Cholesterol (B) Phospholipids (C) Triglycerides (D) Fatty acids 47. Which amino acid contains sulfur? (A) Glycine (B) Cysteine (C) Lysine (D) Alanine 48. The coenzyme required for carboxylation reactions involving COβ fixation is: (A) FAD (B) NADPH (C) Biotin (D) Thiamine 49. The enzyme responsible for the digestion of proteins in the stomach is: (A) Pepsin (B) Trypsin (C) Chymotrypsin (D) Elastase 50. Which molecule is known as the βreducing powerβ in biosynthetic reactions? 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