Photobiophysics – MCQs

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1. Photobiophysics primarily studies:





2. The energy of a photon is given by:





3. Which molecule is the primary light absorber in photosynthesis?





4. The absorption spectrum of chlorophyll shows peaks mainly in:





5. Fluorescence occurs when:





6. The Jablonski diagram is used to explain:





7. Singlet oxygen is:





8. Förster resonance energy transfer (FRET) depends on:





9. Fluorescent proteins like GFP are widely used for:





10. The primary photoreceptor in human vision is:





11. In rhodopsin, light absorption causes:





12. Photobleaching refers to:





13. The quantum yield of fluorescence is defined as:





14. Which photosystem absorbs light at ~680 nm?





15. The photochemical reaction center in Photosystem II is known as:





16. Photodynamic therapy (PDT) uses:





17. The main pigment in rod cells is:





18. Carotenoids in photosynthesis mainly act as:





19. Which law states that the energy of light is proportional to its frequency?





20. Beer–Lambert law relates:





21. In photophysics, the “Stokes shift” refers to:





22. The photochemical event in photosynthesis is:





23. Fluorescence lifetime is measured in:





24. The protein that regenerates 11-cis-retinal in the visual cycle is:





25. In photosystem I, the reaction center absorbs light at:





26. The photoreceptor responsible for color vision is:





27. Which reactive oxygen species is often generated by photosensitizers in PDT?





28. Two-photon excitation microscopy uses:





29. Bioluminescence in fireflies is due to:





30. Retinal is a derivative of:





31. The unit of light intensity is:





32. In fluorescence recovery after photobleaching (FRAP), recovery reflects:





33. Opsins are:





34. The photic zone in aquatic environments refers to:





35. Which light has the highest photon energy?





36. Fluorophores are molecules that:





37. The antenna complex in photosynthesis functions to:





38. Rhodopsin activates which G-protein in vision?





39. Time-resolved fluorescence spectroscopy measures:





40. Photophosphorylation in chloroplasts produces:





41. Which pigment absorbs strongly in the green region and reflects red/blue?





42. Melanin in human skin primarily protects against:





43. Which spectroscopy is widely used in photobiophysics?





44. In photosynthesis, water is split during:





45. The pigment responsible for red fluorescence in corals is:





46. In optogenetics, light is used to:





47. Photosynthetic efficiency is highest under:





48. Which photoreceptor regulates circadian rhythms in mammals?





49. Time-correlated single photon counting (TCSPC) is used to measure:





50. Photobiophysics contributes to medicine mainly through: